Coding

Part:BBa_K1401615:Design

Designed by: Yash Agarwal   Group: iGEM14_BostonU   (2014-10-11)


pConst. - LacI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 11
    Illegal NheI site found at 34
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

All basic BioBricks used were transformed into MoClo variants by adding the following fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. These were then put together using the digestion-ligation MoClo reaction. C0012m has fixed illegal cut sites.



Source

E. Coli

References